CEBPE ELISA Kit

Produktnummer ABIN7830454

Human CEBPE ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CEBPE.

Kurzübersicht für CEBPE ELISA Kit (ABIN7830454)

Target: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CEBPE ELISA Kit

Verwendungszweck: CEBPE (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CEBPE expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CEBPE

Target: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Andere Bezeichnung: CEBPE

Hintergrund:

Synonyms: C/EBP-epsilon|CRP1

Gene Description: CCAAT/enhancer binding protein (C/EBP), epsilon

Gen-ID: 1053

UniProt: Q15744

Pathways: Cellular Response to Molecule of Bacterial Origin