BCAR1 ELISA Kit

Produktnummer ABIN7830438

Das Human BCAR1 ELISA Kit (ABIN7830438) ist ein Colorimetric ELISA Kit zur Detektion von Human BCAR1.

Kurzübersicht für BCAR1 ELISA Kit (ABIN7830438)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails BCAR1 ELISA Kit

Verwendungszweck: BCAR1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BCAR1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Andere Bezeichnung: BCAR1

Hintergrund:

Synonyms: CAS|CAS1|CASS1|CRKAS|P130Cas

Gene Description: breast cancer anti-estrogen resistance 1

Gen-ID: 9564

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling