ATP1A1 ELISA Kit

Produktnummer ABIN7830429

Das Human ATP1A1 ELISA Kit (ABIN7830429) ist ein Colorimetric ELISA Kit zur Detektion von Human ATP1A1.

Kurzübersicht für ATP1A1 ELISA Kit (ABIN7830429)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails ATP1A1 ELISA Kit

Verwendungszweck: ATP1A1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ATP1A1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Andere Bezeichnung: ATP1A1

Hintergrund:

Synonyms: MGC3285|MGC51750

Gene Description: ATPase, Na+/K+ transporting, alpha 1 polypeptide

Gen-ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process