beta Arrestin 1 ELISA Kit

Produktnummer ABIN7830423

Das Human beta Arrestin 1 ELISA Kit (ABIN7830423) ist ein Colorimetric ELISA Kit zur Detektion von Human beta Arrestin 1.

Kurzübersicht für beta Arrestin 1 ELISA Kit (ABIN7830423)

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails beta Arrestin 1 ELISA Kit

Verwendungszweck: ARRB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ARRB1 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu beta Arrestin 1

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Andere Bezeichnung: ARRB1

Hintergrund:

Synonyms: ARB1|ARR1

Gene Description: arrestin, beta 1

Gen-ID: 408

UniProt: P49407

Pathways: Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction