Androgen Receptor ELISA Kit

Produktnummer ABIN7830414

Das Human Androgen Receptor ELISA Kit (ABIN7830414) ist ein Colorimetric ELISA Kit zur Detektion von Human Androgen Receptor.

Kurzübersicht für Androgen Receptor ELISA Kit (ABIN7830414)

Target: Androgen Receptor (AR)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Androgen Receptor ELISA Kit

Verwendungszweck: AR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AR expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Androgen Receptor

Target: Androgen Receptor (AR)

Andere Bezeichnung: AR

Hintergrund:

Synonyms: AIS|DHTR|HUMARA|KD|NR3C4|SBMA|SMAX1|TFM

Gene Description: androgen receptor

Gen-ID: 367

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding