HCP ELISA Kit

Produktnummer ABIN7764597

Dieses Sf9 insect cell line HCP ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Sf9 insect cell line HCP zu quantifizieren.

Kurzübersicht für HCP ELISA Kit (ABIN7764597)

Target: HCP (Host Cell Proteins (HCP))

Reaktivität: Sf9 insect cell line

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Biological Therapeutics, Cell Culture Supernatant, Purified Protein

Produktdetails HCP ELISA Kit

Verwendungszweck: ELISA Kit for the quantitative Measurement of host cell protein (HCP) residues in biopharmaceuticals by Sf9 Insect Cell expression systems

Analytische Methode: Quantitative

Kreuzreaktivität (Details): No cross-reaction exists between the homologous antigens.

Produktmerkmale: Sf9 Insect Cell Host Cell Protein ELISA Kit, G3

Bestandteile:

• 1. Pre-Coated Microplate (Detachable)
• 2. Standard (Stock Solution) -500μg/mL
• 3. Detection antibody (100x)
• 4. TMB Substrates
• 5. Stop Solution
• 6. Wash Solution (100x)
• 7. Diluent Buffer (10x)
• 8. Plate Sealer
• 9. Instruction Manual

Benötigtes Material:

• 10-1000μL pipettor
• Multichannel pipettor
• 1L sterilized deionized water or ultrarpure water
• Sterilized EP tubes
• Absorbent Paper
• Microplate reader
• High-speed centrifuge
• Mini Centrifuge
• Microplate washer or washing bottles
• Data analysis and graphing software

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 3.5 h

Plattentyp: Pre-coated

Protokoll: This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by in putting the ODvalues of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu HCP

Target: HCP (Host Cell Proteins (HCP))

Andere Bezeichnung: Host Cell Proteins