SARS-CoV-2 N-Protein IgM Antibody ELISA Kit

Produktnummer ABIN7666570

Dieses Human ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human zu quantifizieren.

Kurzübersicht für SARS-CoV-2 N-Protein IgM Antibody ELISA Kit (ABIN7666570)

Target: SARS-CoV-2 N-Protein IgM Antibody

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails

Verwendungszweck: anti-SARS-CoV-2 (NP) IgM - ELISA kit for the detection of IgM antibodies to nucleocapsid protein (NP) of coronavirus SARS-CoV-2, causing the COVID-19 disease in human serum and plasma isolated from venous or capillary blood.

Marke: ELISA-VIDITEST

Analytische Methode: Semi-Quantitative

Bestandteile:

• ELISA break-away strips in the handling frame coated with the antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control serum, r.t.u. NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
• 2 mL RF sorbent 2), 25x concentrated RF SORB 25x 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Instruction manual
• Certificate
• of quality 1) r.t.u., ready to use 2) Goat anti-human IgG globulin

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: anti-SARS-CoV-2 (NP) IgM is a solid-phase immunoanalytical test. The specific antigen (Nucleocapsid Protein) is fixed to each well of the microtiter strips. Specific antibodies present in the patient's sample are bound during the first incubation step. After removing unbound material by washing, the presence of the specific antibodies is detected using anti-human IgM conjugate during the second incubation. The unbound peroxidase conjugate is then removed and TMB substrate is added, resulting in the development of a blue colour in positive samples. The enzyme reaction is terminated by addition of the stop solution (colour change to yellow). The intensity of the yellow colour thus developed is proportional to the concentration of antibodies in the sample. 2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: SARS-CoV-2 N-Protein IgM Antibody

Andere Bezeichnung: SARS-CoV-2 NP IgM

Substanzklasse: Antibody