NEFH ELISA Kit

Produktnummer ABIN7666568

Human NEFH ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human NEFH.

Kurzübersicht für NEFH ELISA Kit (ABIN7666568)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cerebrospinal Fluid, Plasma, Serum

Produktdetails NEFH ELISA Kit

Verwendungszweck: pNF-H - ELISA kit for the quantitative determination of phosphorylated forms of heavy neurofilaments.

Marke: ELISA-VIDITEST

Analytische Methode: Quantitative

Bestandteile:

• ELISA doublestrips (16-well strips) in frame with immobilised monoclonal antibody STRIPS Ab 6 strips
• 100 μL pNF-H Standard (bovine pNF-H, 1 μg/mL) STANDARD 2 vial
• s 11 mL anti pNF-H antibody (anti-pNF-H IgY) r.t.u. anti-pNF-H Ab 1 vial
• / 13 mL anti-IgY antibody conjugated to horseradish peroxidase (conjugate anti-IgY/Px) r.t.u. CONJ 1 vial
• 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
• 20 mL Dilution buffer r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate (TMB) r.t.u. TMB-BF 1 vial
• 13 mL Stop solution r.t.u., STOP 1 vial
• Instruction manual
• Certificate
• of quality 1) r.t.u., ready to use

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: pNF-H is a one-step sandwich immunoassay in which the surface of the microtitre plate is coated with the mouse monoclonal antibody that specifically binds the phosphorylated forms of heavy neurofilaments NF-H. The anti-pNF-H rabbit antibodies bind to the thus-bound neurofilament of the test sample, which are subsequently visualized with horseradish peroxidase-labeled anti-IgY antibody. The amount of bound labeled antibodies is determined by color enzymatic reaction. Negative sera do not respond, slight change in the color of the wells is the background of the reaction.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration date of the kit is indicated on the ELISA kit central label, the expiration of reagents is indicated on labels on their package. b. Store unused strips in the sealable pouch and keep the desiccant inside. Store the unused strips in the sealable pouch or close them well into the zipper bag with the desiccant inside. Kits are transported in cooling bags, transport time up to 72 hours has no influence on expiration. If you find any damage at any part of the kit, please inform the manufacturer. c. Store unused test samples undiluted in aliquots and frozen at -18 to -28 °C. Repeated freezing and thawing is not recommended. If you store the serum at +2 to + 10 ° C, then use them within one week. d. Solutions of test samples r.t.u. cannot be stored. Always prepare test samples solutions r.t.u..

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: pNF-H

Substanzklasse: Antibody