C6 ELISA Kit

Produktnummer ABIN7666567

Dieses Human C6 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human C6 zu quantifizieren.

Kurzübersicht für C6 ELISA Kit (ABIN7666567)

Target: C6 (Complement Component 6 (C6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails C6 ELISA Kit

Verwendungszweck: anti-C6 - ELISA kit for detection of antibodies against C6 protein (part of VlsE) in human serum/plasma.

Marke: ELISA-VIDITEST

Analytische Methode: Semi-Quantitative

Bestandteile:

• ELISA break-away strips coated with specific recombinant antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u.1) NC 1 vial
• 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 13 mL Anti-human animal IgM+IgG antibodies labelled with horseradish peroxidase, r.t.u (anti IgM/IgG Px-conjugate) CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate (TMB substrate), r.t.u. (TMB/H2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• .Instruction manual
• Quality control certificate 1) r.t.u. ready to use

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: anti-C6 is a solid-phase immunoanalytical test. The polystyrene strips are coated with the specific antigens. The anti-C6 antibodies, if present in the tested sera, bind to the immobilized antigen. The bonded antibodies react later on with antibodies against human IgM and IgG labelled with horseradish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu C6

Target: C6 (Complement Component 6 (C6))

Andere Bezeichnung: C6

Substanzklasse: Antibody