EBV EA(D) IgA ELISA Kit

Produktnummer ABIN7666557

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human EBV EA(D) IgA.

Kurzübersicht für EBV EA(D) IgA ELISA Kit (ABIN7666557)

Target: EBV EA(D) IgA (Anti-Epstein-Barr Virus Early Antigen (D) IgA (EBV EA(D) IgA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Serum

Produktdetails EBV EA(D) IgA ELISA Kit

Verwendungszweck: anti-EA (D) EBV IgA - EL ISA kit for detection of IgA antibodies to diffusion component of an early antigen (EA (D)) Epstein-Ba rr virus (EBV) in human sera.

Marke: ELISA-VIDITEST

Analytische Methode: Semi-Quantitative

Bestandteile:

• ELISA break-away strips in a frame coated with the s pecific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
• 1.3 mL Positive control serum, r.t.u. CTRL + 1 vial
• 1.3 mL Negative control serum, r.t.u. CTRL - 1 vial
• 13 mL Anti-human IgA antibodies labelled with horseradish peroxidase (anti-IgA Px-conjugate), r.t.u. CONJ 1 vial
• 55 mL Wash buffer concentrate, 10x concentrate d WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Sealable pouch for unused strips Instruction manual
• Quality control certificate 1) ready to use Chromogenic substrate TMB is compatible an d interchangeable between ELISA-VIDITEST kits which contain TMB and not with other Chrom ogenic substrates TMB-O, TMB-BF. 1 http://www.vidia.cz/

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: anti-EA (D) EBV IgA assay i s a solid-phase immunoanalytical test. The polystyrene strips are coated with a recombinant antigen that b ears immunodominant epitopes of EA protein. The anti- EA (D) antibodies, if present in the serum, bind t o the immobilized antigens. The antibodies that do not bind are washed away and those that formed compl exes with the antigens are later on recognised by animal anti-human IgA antibodies labelled with horserad ish peroxidase. The presence of labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu EBV EA(D) IgA

Target: EBV EA(D) IgA (Anti-Epstein-Barr Virus Early Antigen (D) IgA (EBV EA(D) IgA))

Andere Bezeichnung: EA-D Epstein-Barr Virus IgA

Substanzklasse: Antibody