Human FcRn binding Kit (TR-FRET)

Produktnummer ABIN7580958

Kurzübersicht für Human FcRn binding Kit (TR-FRET) (ABIN7580958)

Target: FcRn Inhibitors

Reaktivität: Human

Applikation: Time-resolved Fluorescence Energy Transfer (TR-FRET)

Produktdetails

Detektionsbereich: 0.24 μg/mL-1000 μg/mL

Untere Nachweisgrenze: 0.24 μg/mL

Verwendungszweck: The Human FcRn binding Kit is based on TR-FRET technology to measure the interaction between human FcRn and antibody drug candidates or FcRn inhibitors.

Produktmerkmale: The Human FcRn binding Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to measure the interaction between human FcRn and antibody drug candidates or FcRn inhibitors. It is designed to facilitate the half-life evaluation of antibody drug candidates, high-throughput screening of FcRn inhibitors within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of antibody drugs to bind to FcRn.

Bestandteile: Human FCRN & B2M Heterodimer Protein Europium-chelate
FA labeled human IgG antibody
Human IgG standard
Sample Dilution Buffer
Detection Buffer

Anwendungsinformationen

Kommentare:

• Cost effective - Sufficient quantity at a lower price, accounting for dilution and pipetting losses.
• Comprehensive validation - Validated with various antibody subtypes and antibody drugs.
• Simple and fast operation - No complicated washing steps, significantly reducing time.
• High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
• Accurate and reliable results - High sensitivity with minimal matrix effects.
• High throughput capability - Supports 500 tests, ideal for high-throughput screening.
• Fast completion - Results in just 1 hour.

Probenmenge: 10 μL

Testdauer: 1 h

Protokoll: This Human FcRn binding Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use the mixture of biotinylated FcRn and Europium-chelate labeled streptavidin as the donor, FA labeled Human IgG1 antibody as the acceptor.
In the absence of FcRn-binding components, the donor and acceptor are in close proximity due to the binding of FcRn and FA-labeled Human IgG1 antibody. The donor emits a 620 nm signal upon excitation, which is absorbed by the acceptor, resulting in a 665 nm emission.
In the presence of FcRn-binding components, they interfere with the donor-acceptor interaction, preventing FRET from occurring.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Unopened kit should be stored at 2℃-8℃ upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Antigendetails

Target: FcRn Inhibitors