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CTX-I ELISA Kit (Cross-Linked C-Telopeptides of Type I Collagen) ELISA Kit

CTX-I Reaktivität: Human Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pubmed (15 references)
Produktnummer ABIN6955117
Zzgl. Versandkosten $45.00
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Lieferung in 9 bis 11 Werktagen
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    Competition ELISA
    123.5 pg/mL - 10000 pg/mL
    Untere Nachweisgrenze
    123.5 pg/mL
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CTXI in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    This assay has high sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type I Collagen (CTXI)
    48.4 pg/mL
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
  • Kommentare

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    50 μL
    2 h
    1. Prepare all reagents, samples and standards,
    2. Add 50μL standard or sample to each well.
      Then add 50μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 50μL Stop Solution. Read at 450 nm immediately.
    Aufbereitung der Reagenzien
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 0.5 mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 10,000pg/mL. Please prepare 5 tubes containing 0.6 mL Standard Diluent and produce a triple dilution series according to the picture shown below. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, 123.5pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.


    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
    4. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    5. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    7. Contaminated water or container for reagent preparation will influence the detection result.
    Aufbereitung der Proben
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    4 °C/-20 °C
    Informationen zur Lagerung
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    6 months
  • Xifra, Moreno-Navarrete, Moreno, Ricart, Fernández-Real: "Obesity status influences the relationship among serum osteocalcin, iron stores and insulin sensitivity." in: Clinical nutrition (Edinburgh, Scotland), Vol. 37, Issue 6 Pt A, pp. 2091-2096, (2019) (PubMed).

    Wen, Zhao, Zhao, Wang: "Liraglutide exerts a bone-protective effect in ovariectomized rats with streptozotocin-induced diabetes by inhibiting osteoclastogenesis." in: Experimental and therapeutic medicine, Vol. 15, Issue 6, pp. 5077-5083, (2018) (PubMed).

    Gallego-Escuredo, Lamarca, Villarroya, Domingo, Mateo, Gutierrez, Vidal, Villarroya, Domingo, Giralt: "High FGF21 levels are associated with altered bone homeostasis in HIV-1-infected patients." in: Metabolism: clinical and experimental, Vol. 71, pp. 163-170, (2017) (PubMed).

    Silk, Greene, Baker, Jander: "The effect of calcium and vitamin D supplementation on bone health of male Jockeys." in: Journal of science and medicine in sport, Vol. 20, Issue 3, pp. 225-229, (2016) (PubMed).

    Koole, van Herwaarden, Schalkwijk, Lafeber, Vink, Smeets, Pasterkamp, Moll: "A potential role for glycated cross-links in abdominal aortic aneurysm disease." in: Journal of vascular surgery, Vol. 65, Issue 5, pp. 1493-1503.e3, (2016) (PubMed).

    Engelmann, Wang, Kneitz, Müller-Hilke: "Bone resorption correlates with the frequency of CD5? B cells in the blood of patients with rheumatoid arthritis." in: Rheumatology (Oxford, England), Vol. 54, Issue 3, pp. 545-53, (2015) (PubMed).

    Meister, Engelmann, Kneitz, Müller-Hilke: "The presence of FCGR2B promoter or transmembrane region variant alleles leads to reduced serum IL-6 levels in rheumatoid arthritis." in: Rheumatology international, Vol. 35, Issue 8, pp. 1311-8, (2015) (PubMed).

    Corsetti, Perego, Sansoni, Xu, Barassi, Banfi, Lombardi: "Osteocartilaginous metabolic markers change over a 3-week stage race in pro-cyclists." in: Scandinavian journal of clinical and laboratory investigation, Vol. 75, Issue 6, pp. 523-30, (2015) (PubMed).

    Hamzah, Samarawickrama, Campbell, Pope, Burling, Walker-Bone, Gilleece, Fisher, Post: "Effects of renal tubular dysfunction on bone in tenofovir-exposed HIV-positive patients." in: AIDS, Vol. 29, Issue 14, pp. 1785-92, (2015) (PubMed).

    Yilmaz-Oner, Ozen, Can, Atagunduz, Direskeneli, Inanc: "Biomarkers in Remission According to Different Criteria in Patients with Rheumatoid Arthritis." in: The Journal of rheumatology, Vol. 42, Issue 11, pp. 2066-70, (2015) (PubMed).

    Beranger, Pisani, Castel, Djedaini, Battaglia, Amiaud, Boukhechba, Ailhaud, Michiels, Heymann, Luquet, Amri: "Oxytocin reverses ovariectomy-induced osteopenia and body fat gain." in: Endocrinology, Vol. 155, Issue 4, pp. 1340-52, (2014) (PubMed).

    Krämer, Niemann, Störk, Frantz, Beer, Ertl, Wanner, Weidemann: "Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease." in: The American journal of cardiology, Vol. 114, Issue 6, pp. 895-900, (2014) (PubMed).

    Eschalier, Jean, Pereira, Monzy, Vorilhon, Mactoux, Citron, Sapin, Motreff, Lusson: "Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial." in: Trials, Vol. 13, Issue 1, pp. 25, (2012) (PubMed).

    Fernández-Real, Bulló, Moreno-Navarrete, Ricart, Ros, Estruch, Salas-Salvadó: "A Mediterranean diet enriched with olive oil is associated with higher serum total osteocalcin levels in elderly men at high cardiovascular risk." in: The Journal of clinical endocrinology and metabolism, Vol. 97, Issue 10, pp. 3792-8, (2012) (PubMed).

    Petersen: "[Dentists' drug prescriptions--a recommendation list]." in: Tandlaegebladet, Vol. 90, Issue 7, pp. 306-10, (1986) (PubMed).

  • Target Alle CTX-I ELISA Kits anzeigen
    Andere Bezeichnung
    Cross Linked C-Telopeptide Of Type I Collagen (CTXI) (CTX-I Produkte)
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