SARS-CoV-2 N-Protein IgG Antibody ELISA Kit

Produktnummer ABIN6952759

Das Human SARS-CoV-2 N-Protein IgG Antibody ELISA Kit (ABIN6952759) ist ein Colorimetric ELISA Kit zur Detektion von Human SARS-CoV-2 N-Protein IgG Antibody.

Kurzübersicht für SARS-CoV-2 N-Protein IgG Antibody ELISA Kit (ABIN6952759)

Target: SARS-CoV-2 N-Protein IgG Antibody

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Dried Blood, Plasma, Saliva, Serum

Produktdetails

Verwendungszweck: COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.

Analytische Methode: Semi-Quantitative

Produktmerkmale: COVID-19 N-Protein Human IgG ELISA Kit

Bestandteile:

• SARS-CoV-2 N-protein coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• 5X Sample Diluent
• 1X PBS (DBS version)
• Tween-20 (DBS version)
• Protease Inhibitor Cocktail (DBS version)

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Distilled or deionized water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Testdurchführung:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
3. Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
6. Read at 450 nm immediately.

Ergebnisberechnung:

Calibration curve: Calculate the mean absorbance for each set of duplicate Positive Control (Item C), samples, and subtract the average zero Positive Control optical density. Plot the calibration curve on log-log with Positive Control concentration (Unit/mL) on the xaxis and absorbance on the y-axis using Sigma plot or Excel software.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -80 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: SARS-CoV-2 N-Protein IgG Antibody

Substanzklasse: Antibody

Hintergrund: Gene Symbols: IGG