TLE3 ELISA Kit
Human TLE3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human TLE3 in Cell Culture Supernatant, Cell Samples, Plasma, Serum und Tissue Lysate
Produktnummer ABIN6951703
Kurzübersicht für TLE3 ELISA Kit (ABIN6951703)
Target
Alle TLE3 Produkte
TLE3
(Transducin-Like Enhancer Protein 3 (TLE3))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Verwendungszweck
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Human TLE3 ELISA Kit.
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Analytische Methode
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Quantitative
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Applikationshinweise
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Optimal working dilution should be determined by the investigator.
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Haltbarkeit
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6 months
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TLE3
(Transducin-Like Enhancer Protein 3 (TLE3))
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Andere Bezeichnung
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TLE3
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Gen-ID
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7090
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UniProt
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Q04726
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Pathways
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SARS-CoV-2 Protein Interaktom
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