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TXNL4A ELISA Kit

TXNL4A Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951702
  • Target Alle TXNL4A Produkte
    TXNL4A (Thioredoxin-Like 4A (TXNL4A))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human TXNL4A ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle TXNL4A Produkte
    TXNL4A (Thioredoxin-Like 4A (TXNL4A))
    Andere Bezeichnung
    TXNL4A (TXNL4A Produkte)
    Synonyme
    Txnl4 ELISA Kit, TXNL4A ELISA Kit, txnl4a ELISA Kit, dim1 ELISA Kit, MGC85128 ELISA Kit, DIB1 ELISA Kit, DIM1 ELISA Kit, HsT161 ELISA Kit, SNRNP15 ELISA Kit, TXNL4 ELISA Kit, U5-15kD ELISA Kit, D18Wsu98e ELISA Kit, Dim1 ELISA Kit, ENSMUSG00000057130 ELISA Kit, U5-15kDa ELISA Kit, thioredoxin-like 4A ELISA Kit, thioredoxin like 4A ELISA Kit, thioredoxin like 4A S homeolog ELISA Kit, Txnl4a ELISA Kit, TXNL4A ELISA Kit, txnl4a ELISA Kit, LOC664328 ELISA Kit, txnl4a.S ELISA Kit
    Gen-ID
    10907
    UniProt
    P83876
    Pathways
    Ribonucleoprotein Complex Subunit Organization
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