SRPK1 ELISA Kit

Produktnummer ABIN6951698

Produktdetails SRPK1 ELISA Kit

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human SRPK1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu SRPK1

Target: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Andere Bezeichnung: SRPK1 (SRPK1 Produkte)

Synonyme: SFRSK1 ELISA Kit, AU017960 ELISA Kit, BEST:CK01209 ELISA Kit, CG11489 ELISA Kit, CG9085 ELISA Kit, CK01209 ELISA Kit, Dmel\\CG11489 ELISA Kit, SRPK ELISA Kit, SRPK1 ELISA Kit, Srpk79D ELISA Kit, atc ELISA Kit, srpk1 ELISA Kit, zgc:63789 ELISA Kit, wu:fc06h03 ELISA Kit, zgc:158800 ELISA Kit, sfrsk1 ELISA Kit, SRSF protein kinase 1 ELISA Kit, serine/arginine-rich protein specific kinase 1 ELISA Kit, Serine-arginine protein kinase at 79D ELISA Kit, SRSF protein kinase 1a ELISA Kit, SRSF protein kinase 1b ELISA Kit, SRSF protein kinase 1 S homeolog ELISA Kit, SRPK1 ELISA Kit, Srpk1 ELISA Kit, Srpk79D ELISA Kit, srpk1a ELISA Kit, srpk1b ELISA Kit, srpk1.S ELISA Kit

Gen-ID: 6732

UniProt: Q96SB4

Pathways: Toll-Like Receptors Cascades