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NCKIPSD ELISA Kit

NCKIPSD Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951696
  • Target Alle NCKIPSD ELISA Kits anzeigen
    NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human NCKIPSD ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle NCKIPSD ELISA Kits anzeigen
    NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))
    Andere Bezeichnung
    NCKIPSD (NCKIPSD Produkte)
    Synonyme
    AF3P21 ELISA Kit, DIP ELISA Kit, DIP1 ELISA Kit, ORF1 ELISA Kit, SPIN90 ELISA Kit, VIP54 ELISA Kit, WASLBP ELISA Kit, WISH ELISA Kit, Wasbp ELISA Kit, SH3 adapter protein SPIN90 ELISA Kit, NCK interacting protein with SH3 domain ELISA Kit, CpipJ_CPIJ019280 ELISA Kit, NCKIPSD ELISA Kit, Nckipsd ELISA Kit
    Gen-ID
    51517
    UniProt
    Q9NZQ3
    Pathways
    Protein targeting to Nucleus
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