C1orf33 ELISA Kit

Produktnummer ABIN6951676

Produktdetails C1orf33 ELISA Kit

Target: C1orf33 (MRTO4) (mRNA Turnover 4 Homolog (MRTO4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human MRTO4 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu C1orf33

Target: C1orf33 (MRTO4) (mRNA Turnover 4 Homolog (MRTO4))

Andere Bezeichnung: MRTO4 (MRTO4 Produkte)

Synonyme: mrt4 ELISA Kit, wu:fb71a02 ELISA Kit, zgc:110388 ELISA Kit, C1orf33 ELISA Kit, MRT4 ELISA Kit, dJ657E11.4 ELISA Kit, RGD1311709 ELISA Kit, 2610012O22Rik ELISA Kit, Mg684 ELISA Kit, Mrt4 ELISA Kit, MRT4 homolog, ribosome maturation factor L homeolog ELISA Kit, MRT4 homolog, ribosome maturation factor ELISA Kit, mRNA turnover 4, ribosome maturation factor ELISA Kit, mrto4.L ELISA Kit, mrto4 ELISA Kit, MRTO4 ELISA Kit, Mrto4 ELISA Kit

Gen-ID: 51154

UniProt: Q9UKD2