MOBKL1A ELISA Kit

Produktnummer ABIN6951675

Produktdetails MOBKL1A ELISA Kit

Target: MOBKL1A (MOB1B) (MOB Kinase Activator 1B (MOB1B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human MOB1B ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu MOBKL1A

Target: MOBKL1A (MOB1B) (MOB Kinase Activator 1B (MOB1B))

Andere Bezeichnung: MOB1B (MOB1B Produkte)

Synonyme: MATS2 ELISA Kit, MOB4A ELISA Kit, MOBKL1A ELISA Kit, 1110003E08Rik ELISA Kit, AU015450 ELISA Kit, B230364F10 ELISA Kit, Mobkl1a ELISA Kit, mobkl1a ELISA Kit, mobkl1aa ELISA Kit, wu:fj65h03 ELISA Kit, zgc:56156 ELISA Kit, zgc:77872 ELISA Kit, MOB1B ELISA Kit, mob4a ELISA Kit, mats2 ELISA Kit, C2orf6 ELISA Kit, MOB1 ELISA Kit, MOBK1B ELISA Kit, Mob4B ELISA Kit, cb512 ELISA Kit, mobkl1ab ELISA Kit, sb:cb512 ELISA Kit, zgc:111840 ELISA Kit, MOB kinase activator 1B ELISA Kit, MOB kinase activator 1Ba ELISA Kit, Mps one binder kinase activator-like 1A ELISA Kit, mps one binder kinase activator-like 1A ELISA Kit, MOB kinase activator 1B L homeolog ELISA Kit, MOB kinase activator 1Bb ELISA Kit, MOB1B ELISA Kit, Mob1b ELISA Kit, mob1ba ELISA Kit, mol1a ELISA Kit, LOC100282811 ELISA Kit, LOC100285953 ELISA Kit, mob1b ELISA Kit, mob1b.L ELISA Kit, mob1bb ELISA Kit

Gen-ID: 92597

UniProt: Q7L9L4