×
Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.
MOBKL1A ELISA Kit
Dieses Human MOBKL1A ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human MOBKL1A in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate zu quantifizieren.
Produktnummer ABIN6951675
Kurzübersicht für MOBKL1A ELISA Kit (ABIN6951675)
Target
Alle MOBKL1A (MOB1B) ELISA Kits anzeigen
MOBKL1A (MOB1B)
(MOB Kinase Activator 1B (MOB1B))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktdetails MOBKL1A ELISA Kit
(ausblenden)
Verwendungszweck
Human MOB1B ELISA Kit.
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
(anzeigen)
Anwendungsinformationen
(ausblenden)
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
(ausblenden)
Haltbarkeit
6 months
Details zu MOBKL1A
(ausblenden)
MOBKL1A (MOB1B)
(MOB Kinase Activator 1B (MOB1B))
Andere Bezeichnung
MOB1B
Gen-ID
92597
UniProt
Q7L9L4
Zuletzt angesehen
(ausblenden)
Chat with us , powered by LiveChat