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CD57 ELISA Kit

B3GAT1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951578
  • Target Alle CD57 (B3GAT1) ELISA Kits anzeigen
    CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))
    Reaktivität
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human CD57/B3gat1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product B3GAT1 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle CD57 (B3GAT1) ELISA Kits anzeigen
    CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))
    Andere Bezeichnung
    CD57 (B3gat1 (B3GAT1 Produkte)
    Synonyme
    CD57 ELISA Kit, GLCATP ELISA Kit, GLCUATP ELISA Kit, HNK1 ELISA Kit, LEU7 ELISA Kit, NK-1 ELISA Kit, NK1 ELISA Kit, 0710007K08Rik ELISA Kit, AI846286 ELISA Kit, GlcAT-P ELISA Kit, Glcatp ELISA Kit, Hnk1 ELISA Kit, Hnk-1 ELISA Kit, b3gat1 ELISA Kit, si:dkey-22o20.1 ELISA Kit, cd57 ELISA Kit, glcatp ELISA Kit, glcuatp ELISA Kit, hnk-1 ELISA Kit, hnk1 ELISA Kit, leu7 ELISA Kit, nk-1 ELISA Kit, nk1 ELISA Kit, beta-1,3-glucuronyltransferase 1 ELISA Kit, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) ELISA Kit, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) a ELISA Kit, beta-1,3-glucuronyltransferase 1 L homeolog ELISA Kit, B3GAT1 ELISA Kit, B3gat1 ELISA Kit, b3gat1a ELISA Kit, b3gat1.L ELISA Kit
    Gen-ID
    27087
    UniProt
    Q9P2W7
    Pathways
    Glycosaminoglycan Metabolic Process
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