LAP3 ELISA Kit

Produktnummer ABIN6951571

Produktdetails LAP3 ELISA Kit

Target: LAP3 (Cytosol Aminopeptidase (LAP3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human LAP3 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu LAP3

Target: LAP3 (Cytosol Aminopeptidase (LAP3))

Andere Bezeichnung: LAP3 (LAP3 Produkte)

Synonyme: LAP ELISA Kit, LAPEP ELISA Kit, PEPS ELISA Kit, 2410015L10Rik ELISA Kit, AA410100 ELISA Kit, LAP-3 ELISA Kit, Lap ELISA Kit, Lapep ELISA Kit, Pep-7 ELISA Kit, Pep-S ELISA Kit, Pep7 ELISA Kit, Peps ELISA Kit, cytosol aminopeptidase ELISA Kit, leucine aminopeptidase 3 ELISA Kit, pepA ELISA Kit, Plabr_1567 ELISA Kit, Dester_0800 ELISA Kit, Weevi_0186 ELISA Kit, Marky_0570 ELISA Kit, Halhy_2623 ELISA Kit, FsymDg_3217 ELISA Kit, Flexsi_2186 ELISA Kit, Runsl_3980 ELISA Kit, lap3 ELISA Kit, LAP3 ELISA Kit, Lap3 ELISA Kit

Gen-ID: 51056

UniProt: P28838