Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

HSD17B14 IQ-ELISA Kit

Human HSD17B14 ELISA-Kit qPCR-Assay zur Quantifizierung von Human HSD17B14 in Cell Culture Supernatant, Plasma und Serum
Produktnummer ABIN6951568

Kurzübersicht für HSD17B14 IQ-ELISA Kit (ABIN6951568)

Target

Alle HSD17B14 IQ-ELISA Kits anzeigen
HSD17B14 (Hydroxysteroid (17-Beta) Dehydrogenase 14 (HSD17B14))

Reaktivität

  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Nachweismethode

qPCR

Methodentyp

DNA-Binding ELISA

Detektionsbereich

1.22 pg/mL - 5000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma, Serum
  • Untere Nachweisgrenze

    1.22 pg/mL

    Verwendungszweck

    Human Immunoquantitative (PCR-Based) HSD17B14 ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Analytische Methode

    Quantitative

    Bestandteile

    • HSD17B14 Microplate (Item A): 96 well PCR plate coated with anti-Human HSD17B14
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Human HSD17B14
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for HSD17B14 (Item F): 2 vials of a 4x concentrated solution of anti-Human HSD17B14 affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

    Benötigtes Material

    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR

    Aufbereitung der Reagenzien

    Recommended Dilution for serum and plasma samples2 - 20 fold

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit

    6 months
  • Target Alle HSD17B14 IQ-ELISA Kits anzeigen

    HSD17B14 (Hydroxysteroid (17-Beta) Dehydrogenase 14 (HSD17B14))

    Andere Bezeichnung

    HSD17B14

    Gen-ID

    51171

    UniProt

    Q9BPX1
Sie sind hier:
Chat with us!