PPIL1 ELISA Kit
This anti-PPIL1 antibody (ABIN6951473) is a antibody detecting PPIL1 in ELISA. Suitable for Human.
Produktnummer ABIN6951473
Kurzübersicht für PPIL1 ELISA Kit (ABIN6951473)
Target
Alle PPIL1 ELISA Kits anzeigen
PPIL1
(Peptidylprolyl Isomerase (Cyclophilin)-Like 1 (PPIL1))
Reaktivität
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Verwendungszweck
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Human PPIL1 ELISA Kit.
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Analytische Methode
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Quantitative
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Applikationshinweise
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Optimal working dilution should be determined by the investigator.
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Haltbarkeit
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6 months
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PPIL1
(Peptidylprolyl Isomerase (Cyclophilin)-Like 1 (PPIL1))
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Andere Bezeichnung
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PPIL1
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Gen-ID
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51645
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UniProt
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Q9Y3C6
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