DDOST ELISA Kit

Produktnummer ABIN6951433

Produktdetails DDOST ELISA Kit

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human DDOST ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu DDOST

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Andere Bezeichnung: DDOST (DDOST Produkte)

Synonyme: AGER1 ELISA Kit, CDG1R ELISA Kit, OKSWcl45 ELISA Kit, OST ELISA Kit, OST48 ELISA Kit, WBP1 ELISA Kit, DDOST ELISA Kit, wu:fa11d01 ELISA Kit, zgc:66068 ELISA Kit, zgc:77478 ELISA Kit, Ddost ELISA Kit, CG9022 ELISA Kit, DmOST50 ELISA Kit, Dmel\\CG9022 ELISA Kit, DrOST ELISA Kit, OST48/WBP1 ELISA Kit, Ots48 ELISA Kit, anon-EST:Liang-1.11 ELISA Kit, clone 1.11 ELISA Kit, ost48 ELISA Kit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit ELISA Kit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) S homeolog ELISA Kit, dolichyl-diphosphooligosaccharide-protein glycosyltransferase ELISA Kit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) ELISA Kit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit ELISA Kit, dolichyl-di-phosphooligosaccharide-protein glycotransferase ELISA Kit, Oligosaccharyltransferase 48kD subunit ELISA Kit, DDOST ELISA Kit, ddost.S ELISA Kit, GTR26 ELISA Kit, ddost ELISA Kit, Ddost ELISA Kit, LOC5580056 ELISA Kit, PTRG_01946 ELISA Kit, Ost48 ELISA Kit

Gen-ID: 1650

UniProt: P39656

Pathways: Cell RedoxHomeostasis