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PPIL2 ELISA Kit

PPIL2 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951429
  • Target Alle PPIL2 Produkte
    PPIL2 (Peptidylprolyl Isomerase (Cyclophilin)-Like 2 (PPIL2))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human PPIL2 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle PPIL2 Produkte
    PPIL2 (Peptidylprolyl Isomerase (Cyclophilin)-Like 2 (PPIL2))
    Andere Bezeichnung
    PPIL2 (PPIL2 Produkte)
    Synonyme
    cyc4 ELISA Kit, cyp60 ELISA Kit, hcyp-60 ELISA Kit, CYC4 ELISA Kit, CYP60 ELISA Kit, Cyp-60 ELISA Kit, UBOX7 ELISA Kit, hCyP-60 ELISA Kit, 0610009L05Rik ELISA Kit, 1700016N17Rik ELISA Kit, 4921520K19Rik ELISA Kit, 4930511F14Rik ELISA Kit, AA589416 ELISA Kit, C130078A06Rik ELISA Kit, si:dkeyp-86g2.1 ELISA Kit, zgc:56616 ELISA Kit, zgc:86735 ELISA Kit, peptidylprolyl isomerase like 2 S homeolog ELISA Kit, peptidylprolyl isomerase like 2 ELISA Kit, peptidylprolyl isomerase (cyclophilin)-like 2 ELISA Kit, ppil2.S ELISA Kit, PPIL2 ELISA Kit, ppil2 ELISA Kit, Ppil2 ELISA Kit
    Gen-ID
    23759
    UniProt
    Q13356
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