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BCL6 ELISA Kit

BCL6 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951387
  • Target Alle BCL6 ELISA Kits anzeigen
    BCL6 (B-Cell CLL/lymphoma 6 (BCL6))
    Reaktivität
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human BCL6 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle BCL6 ELISA Kits anzeigen
    BCL6 (B-Cell CLL/lymphoma 6 (BCL6))
    Andere Bezeichnung
    BCL6 (BCL6 Produkte)
    Synonyme
    bcl5 ELISA Kit, laz3 ELISA Kit, bcl6a ELISA Kit, znf51 ELISA Kit, zbtb27 ELISA Kit, MGC132260 ELISA Kit, Bcl5 ELISA Kit, BCL5 ELISA Kit, BCL6A ELISA Kit, LAZ3 ELISA Kit, ZBTB27 ELISA Kit, ZNF51 ELISA Kit, B-cell CLL/lymphoma 6 L homeolog ELISA Kit, B-cell CLL/lymphoma 6 ELISA Kit, B cell leukemia/lymphoma 6 ELISA Kit, bcl6.L ELISA Kit, bcl6 ELISA Kit, BCL6 ELISA Kit, Bcl6 ELISA Kit
    Gen-ID
    604
    UniProt
    P41182
    Pathways
    Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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