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AARSD1 ELISA Kit

AARSD1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951364
  • Target Alle AARSD1 ELISA Kits anzeigen
    AARSD1 (Alanyl-tRNA Synthetase Domain Containing 1 (AARSD1))
    Reaktivität
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human AARSD1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle AARSD1 ELISA Kits anzeigen
    AARSD1 (Alanyl-tRNA Synthetase Domain Containing 1 (AARSD1))
    Andere Bezeichnung
    AARSD1 (AARSD1 Produkte)
    Synonyme
    im:7152658 ELISA Kit, zgc:101066 ELISA Kit, AARSD1 ELISA Kit, 1110069E20Rik ELISA Kit, 2310044P18Rik ELISA Kit, AA589600 ELISA Kit, AlaX ELISA Kit, AlaXp-II ELISA Kit, aarsd1-a ELISA Kit, aarsd1-b ELISA Kit, prostaglandin E synthase 3 (cytosolic)-like ELISA Kit, alanyl-tRNA synthetase domain containing 1 ELISA Kit, alanyl-tRNA synthetase domain containing 1 L homeolog ELISA Kit, PTGES3L ELISA Kit, aarsd1 ELISA Kit, AARSD1 ELISA Kit, Aarsd1 ELISA Kit, aarsd1.L ELISA Kit
    UniProt
    Q9BTE6
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