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Tetraspanin 7 ELISA Kit

TSPAN7 Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951346
  • Target Alle Tetraspanin 7 (TSPAN7) ELISA Kits anzeigen
    Tetraspanin 7 (TSPAN7)
    Reaktivität
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Mouse TSPAN7 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle Tetraspanin 7 (TSPAN7) ELISA Kits anzeigen
    Tetraspanin 7 (TSPAN7)
    Andere Bezeichnung
    TSPAN7 (TSPAN7 Produkte)
    Synonyme
    A15 ELISA Kit, CCG-B7 ELISA Kit, CD231 ELISA Kit, DXS1692E ELISA Kit, MRX58 ELISA Kit, MXS1 ELISA Kit, TALLA-1 ELISA Kit, TM4SF2 ELISA Kit, TM4SF2b ELISA Kit, 1200014P11Rik ELISA Kit, AI323365 ELISA Kit, Cd231 ELISA Kit, Mxs1 ELISA Kit, PE31 ELISA Kit, R74651 ELISA Kit, TALLA ELISA Kit, Tm4sf2 ELISA Kit, tm4sf2 ELISA Kit, zgc:63823 ELISA Kit, wu:fa11g01 ELISA Kit, wu:fd18e11 ELISA Kit, wu:fl39g07 ELISA Kit, a15 ELISA Kit, ccg-b7 ELISA Kit, cd231 ELISA Kit, mrx58 ELISA Kit, mxs1 ELISA Kit, talla-1 ELISA Kit, tspan7 ELISA Kit, tetraspanin 7 ELISA Kit, tetraspanin 7 L homeolog ELISA Kit, TSPAN7 ELISA Kit, Tspan7 ELISA Kit, tspan7 ELISA Kit, tspan7.L ELISA Kit
    Gen-ID
    21912
    UniProt
    Q62283
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