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CADM2 ELISA Kit

CADM2 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951339
  • Target Alle CADM2 ELISA Kits anzeigen
    CADM2 (Cell Adhesion Molecule 2 (CADM2))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human CADM2 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle CADM2 ELISA Kits anzeigen
    CADM2 (Cell Adhesion Molecule 2 (CADM2))
    Andere Bezeichnung
    CADM2 (CADM2 Produkte)
    Synonyme
    2900078E11Rik ELISA Kit, 9330131D06 ELISA Kit, A830029E02Rik ELISA Kit, Igdf4d ELISA Kit, Igsf4d ELISA Kit, NECL3 ELISA Kit, SynCAM 2 ELISA Kit, SynCAM2 ELISA Kit, Necl-3 ELISA Kit, IGSF4D ELISA Kit, synCAM2 ELISA Kit, igsf4d ELISA Kit, zgc:66339 ELISA Kit, cell adhesion molecule 2 ELISA Kit, cell adhesion molecule 2a ELISA Kit, CADM2 ELISA Kit, Cadm2 ELISA Kit, cadm2a ELISA Kit
    Gen-ID
    253559
    UniProt
    Q8N3J6
    Pathways
    Cell-Cell Junction Organization
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