SCG2 ELISA Kit
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- Target Alle SCG2 ELISA Kits anzeigen
- SCG2 (Secretogranin II (SCG2))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Human SCG2 ELISA Kit.
- Proben
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytische Methode
- Quantitative
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product SCG2 ELISA Kit
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Haltbarkeit
- 6 months
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- Target Alle SCG2 ELISA Kits anzeigen
- SCG2 (Secretogranin II (SCG2))
- Andere Bezeichnung
- SCG2 (SCG2 Produkte)
- Synonyme
- LOC397939 ELISA Kit, MGC84703 ELISA Kit, scg2 ELISA Kit, wu:fj34e01 ELISA Kit, zgc:153925 ELISA Kit, MGC145924 ELISA Kit, SCG2 ELISA Kit, SgII ELISA Kit, DKFZp459O193 ELISA Kit, CHGC ELISA Kit, EM66 ELISA Kit, SN ELISA Kit, Chcg ELISA Kit, Chgc ELISA Kit, SCGG2 ELISA Kit, secretogranin II L homeolog ELISA Kit, secretogranin II (chromogranin C), b ELISA Kit, secretogranin II ELISA Kit, Secretogranin-2 ELISA Kit, scg2.L ELISA Kit, scg2b ELISA Kit, scg2 ELISA Kit, SCG2 ELISA Kit, Scg2 ELISA Kit
- Gen-ID
- 7857
- UniProt
- P13521
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