SHPK ELISA Kit

Produktnummer ABIN6951296

Produktdetails SHPK ELISA Kit

Target: SHPK (Sedoheptulokinase (SHPK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human SHPK ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu SHPK

Target: SHPK (Sedoheptulokinase (SHPK))

Andere Bezeichnung: SHPK (SHPK Produkte)

Synonyme: SHPK ELISA Kit, CARKL ELISA Kit, carkl ELISA Kit, MGC154818 ELISA Kit, SHK ELISA Kit, 4930431K22Rik ELISA Kit, AI194947 ELISA Kit, AW260459 ELISA Kit, Carkl ELISA Kit, sedoheptulokinase ELISA Kit, sedoheptulokinase S homeolog ELISA Kit, Sedoheptulokinase ELISA Kit, SHPK ELISA Kit, shpk.S ELISA Kit, Plabr_2171 ELISA Kit, shpk ELISA Kit, Shpk ELISA Kit

Gen-ID: 23729

UniProt: Q9UHJ6

Pathways: Cellular Response to Molecule of Bacterial Origin