TNFAIP8 ELISA Kit

Produktnummer ABIN6951264

Produktdetails TNFAIP8 ELISA Kit

Target: TNFAIP8 (Tumor Necrosis Factor, alpha-Induced Protein 8 (TNFAIP8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human TNFAIP8 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu TNFAIP8

Target: TNFAIP8 (Tumor Necrosis Factor, alpha-Induced Protein 8 (TNFAIP8))

Andere Bezeichnung: TNFAIP8 (TNFAIP8 Produkte)

Synonyme: MGC55331 ELISA Kit, zgc:55331 ELISA Kit, si:dkey-49m19.6 ELISA Kit, GG2-1 ELISA Kit, MDC-3.13 ELISA Kit, SCC-S2 ELISA Kit, SCCS2 ELISA Kit, AA987150 ELISA Kit, E130304C20Rik ELISA Kit, ENSMUSG00000073567 ELISA Kit, Gg2-1 ELISA Kit, Gm10539 ELISA Kit, Nded ELISA Kit, Ssc-2 ELISA Kit, tumor necrosis factor, alpha-induced protein 8-like 1 ELISA Kit, TNF alpha induced protein 8 S homeolog ELISA Kit, TNF alpha induced protein 8 ELISA Kit, tumor necrosis factor, alpha-induced protein 8 ELISA Kit, tnfaip8l1 ELISA Kit, tnfaip8.S ELISA Kit, TNFAIP8 ELISA Kit, Tnfaip8 ELISA Kit

Gen-ID: 25816

UniProt: O95379