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SCLY ELISA Kit

SCLY Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951261
  • Target Alle SCLY ELISA Kits anzeigen
    SCLY (Selenocysteine Lyase (SCLY))
    Reaktivität
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human SCLY ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle SCLY ELISA Kits anzeigen
    SCLY (Selenocysteine Lyase (SCLY))
    Andere Bezeichnung
    SCLY (SCLY Produkte)
    Synonyme
    SCL ELISA Kit, hSCL ELISA Kit, 9830169H08 ELISA Kit, A930015N15Rik ELISA Kit, Scly1 ELISA Kit, Scly2 ELISA Kit, zgc:171514 ELISA Kit, selenocysteine lyase ELISA Kit, selenocysteine lyase L homeolog ELISA Kit, Selenocysteine lyase ELISA Kit, SCLY ELISA Kit, Scly ELISA Kit, scly.L ELISA Kit, csdB ELISA Kit, EUBELI_20129 ELISA Kit, Deima_1599 ELISA Kit, scly ELISA Kit
    Gen-ID
    50880
    UniProt
    Q9JLI6
    Pathways
    Stem Cell Maintenance
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