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PDZD11 ELISA Kit

PDZD11 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951209
  • Target Alle PDZD11 Produkte
    PDZD11 (PDZ Domain Containing 11 (PDZD11))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human PDZD11 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle PDZD11 Produkte
    PDZD11 (PDZ Domain Containing 11 (PDZD11))
    Andere Bezeichnung
    PDZD11 (PDZD11 Produkte)
    Synonyme
    RGD1560007 ELISA Kit, MGC89018 ELISA Kit, AIPP1 ELISA Kit, PDZK11 ELISA Kit, PISP ELISA Kit, 1810012H22Rik ELISA Kit, 2310079D11Rik ELISA Kit, 2700099C19Rik ELISA Kit, AI854765 ELISA Kit, Pdzk11 ELISA Kit, zgc:77536 ELISA Kit, PDZ domain containing 11 ELISA Kit, PDZ domain containing 11 L homeolog ELISA Kit, Pdzd11 ELISA Kit, pdzd11.L ELISA Kit, pdzd11 ELISA Kit, PDZD11 ELISA Kit
    Gen-ID
    51248
    UniProt
    Q5EBL8
    Pathways
    SARS-CoV-2 Protein Interaktom
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