CNDP2 ELISA Kit

Produktnummer ABIN6951100

Produktdetails CNDP2 ELISA Kit

Target: CNDP2 (CNDP Dipeptidase 2 (Metallopeptidase M20 Family) (CNDP2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse CNDP2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu CNDP2

Target: CNDP2 (CNDP Dipeptidase 2 (Metallopeptidase M20 Family) (CNDP2))

Andere Bezeichnung: CNDP2 (CNDP2 Produkte)

Synonyme: 0610010E05Rik ELISA Kit, C76600 ELISA Kit, Cn2 ELISA Kit, Dip-2 ELISA Kit, Pep-1 ELISA Kit, Pep1 ELISA Kit, CN2 ELISA Kit, CPGL ELISA Kit, HsT2298 ELISA Kit, PEPA ELISA Kit, cn2 ELISA Kit, cpgl ELISA Kit, darmin-r ELISA Kit, CNDP2 ELISA Kit, zgc:92569 ELISA Kit, wu:fd20d11 ELISA Kit, wu:fe17f09 ELISA Kit, cndp2b ELISA Kit, PEPC1 ELISA Kit, PEPCase ELISA Kit, PPC1 ELISA Kit, PPEPC4 ELISA Kit, pepc ELISA Kit, carnosine dipeptidase 2 ELISA Kit, CNDP dipeptidase 2 (metallopeptidase M20 family) ELISA Kit, CNDP dipeptidase 2 (metallopeptidase M20 family) S homeolog ELISA Kit, CNDP dipeptidase 2 (metallopeptidase M20 family) L homeolog ELISA Kit, phosphoenolpyruvate carboxylase 1 ELISA Kit, Cndp2 ELISA Kit, CNDP2 ELISA Kit, cndp2 ELISA Kit, cndp2.S ELISA Kit, cndp2.L ELISA Kit, pep1 ELISA Kit

Gen-ID: 55748

UniProt: Q96KP4