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Parvin alpha ELISA Kit

PARVA Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951071
  • Target Alle Parvin alpha (PARVA) Produkte
    Parvin alpha (PARVA) (Parvin, alpha (PARVA))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human PARVA ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle Parvin alpha (PARVA) Produkte
    Parvin alpha (PARVA) (Parvin, alpha (PARVA))
    Andere Bezeichnung
    PARVA (PARVA Produkte)
    Synonyme
    CH-ILKBP ELISA Kit, MXRA2 ELISA Kit, Actp ELISA Kit, 2010012A22Rik ELISA Kit, 5430400F08Rik ELISA Kit, AI225929 ELISA Kit, AU042898 ELISA Kit, Parvin ELISA Kit, parva ELISA Kit, wu:fc59e06 ELISA Kit, zgc:101643 ELISA Kit, PARVA ELISA Kit, Parva ELISA Kit, parvin alpha ELISA Kit, parvin, alpha ELISA Kit, parvin, alpha a ELISA Kit, PARVA ELISA Kit, Parva ELISA Kit, parvaa ELISA Kit
    Gen-ID
    55742
    UniProt
    Q9NVD7
    Pathways
    Smooth Muscle Cell Migration
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