ATOX1 ELISA Kit

Produktnummer ABIN6951064

Produktdetails ATOX1 ELISA Kit

Target: ATOX1 (ATX1 Antioxidant Protein 1 Homolog (ATOX1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human ATOX1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu ATOX1

Target: ATOX1 (ATX1 Antioxidant Protein 1 Homolog (ATOX1))

Andere Bezeichnung: ATOX1 (ATOX1 Produkte)

Synonyme: ATX1 ELISA Kit, HAH1 ELISA Kit, AI256639 ELISA Kit, Atx1 ELISA Kit, CG12562 ELISA Kit, CG32446 ELISA Kit, DmAtox1 ELISA Kit, Dmel\\CG32446 ELISA Kit, atx1 ELISA Kit, hah1 ELISA Kit, DDBDRAFT_0185901 ELISA Kit, DDBDRAFT_0266724 ELISA Kit, DDB_0185901 ELISA Kit, DDB_0266724 ELISA Kit, SAH ELISA Kit, ATOX1 ELISA Kit, antioxidant 1 copper chaperone ELISA Kit, Antioxidant 1 copper chaperone ELISA Kit, copper transport protein ELISA Kit, ATX1 antioxidant protein 1 homolog ELISA Kit, ATOX1 ELISA Kit, Atox1 ELISA Kit, atox1 ELISA Kit

Gen-ID: 475

UniProt: O00244

Pathways: Transition Metal Ion Homeostasis