Adenylate Kinase 1 ELISA Kit

Produktnummer ABIN6951056

Produktdetails Adenylate Kinase 1 ELISA Kit

Target: Adenylate Kinase 1 (AK1)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Rat AK1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu Adenylate Kinase 1

Target: Adenylate Kinase 1 (AK1)

Andere Bezeichnung: AK1 (AK1 Produkte)

Synonyme: ADK-1 ELISA Kit, AK1 ELISA Kit, CG17146 ELISA Kit, DAK1 ELISA Kit, Dak1 ELISA Kit, Dmel\\CG17146 ELISA Kit, adk1 ELISA Kit, bs34e10.y1 ELISA Kit, ak5 ELISA Kit, ADENYLATE KINASE 1 ELISA Kit, MLE2.3 ELISA Kit, MLE2_3 ELISA Kit, adenylate kinase 1 ELISA Kit, Ak-1 ELISA Kit, B430205N08Rik ELISA Kit, Ak 1 ELISA Kit, zgc:91930 ELISA Kit, Myokinase ELISA Kit, Adenylate kinase 1 ELISA Kit, adenylate kinase 1 ELISA Kit, Adk1 ELISA Kit, ak1 ELISA Kit, AK1 ELISA Kit, ADK1 ELISA Kit, Ak1 ELISA Kit

Gen-ID: 24183

UniProt: P39069

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process