beta Defensin 1 ELISA Kit

Produktnummer ABIN6810507

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human, Maus und Ratte beta Defensin 1 in Cell Culture Supernatant und Serum.

Kurzübersicht für beta Defensin 1 ELISA Kit (ABIN6810507)

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reaktivität: Human, Maus, Ratte

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.1 ng/mL - 1000 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails beta Defensin 1 ELISA Kit

Untere Nachweisgrenze: 0.1 ng/mL

Verwendungszweck: Human/Mouse/Rat Beta-defensin 1 EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.

Analytische Methode: Quantitative

Spezifität: This EIA kit is designed to detect human, mouse, and rat Beta-defensin 1

Sensitivität: 0.9 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Aufbereitung der Reagenzien:

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Haltbarkeit: 6 months

Details zu beta Defensin 1

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Andere Bezeichnung: Beta-defensin 1

Gen-ID: 1672

UniProt: P60022