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beta Defensin 1 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human, Maus und Ratte beta Defensin 1 in Cell Culture Supernatant und Serum.
Produktnummer ABIN6810507

Kurzübersicht für beta Defensin 1 ELISA Kit (ABIN6810507)

Target

Alle beta Defensin 1 (DEFB1) ELISA Kits anzeigen
beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Reaktivität

  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Nachweismethode

Colorimetric

Methodentyp

Competition ELISA

Detektionsbereich

0.1 ng/mL - 1000 ng/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum
  • Untere Nachweisgrenze

    0.1 ng/mL

    Verwendungszweck

    Human/Mouse/Rat Beta-defensin 1 EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.

    Analytische Methode

    Quantitative

    Spezifität

    This EIA kit is designed to detect human, mouse, and rat Beta-defensin 1

    Sensitivität

    0.9 ng/mL

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Protokoll

    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.

    Aufbereitung der Reagenzien

    Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

    Haltbarkeit

    6 months
  • Target Alle beta Defensin 1 (DEFB1) ELISA Kits anzeigen

    beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

    Andere Bezeichnung

    Beta-defensin 1

    Gen-ID

    1672

    UniProt

    P60022
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