AMBP ELISA Kit

Produktnummer ABIN6730831

Produktdetails AMBP ELISA Kit

Target: AMBP (alpha 1 Microglobulin/bikunin precursor (AMBP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Alpha-1 Microglobulin/AMBP ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Alpha-1 Microglobulin.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu AMBP

Target: AMBP (alpha 1 Microglobulin/bikunin precursor (AMBP))

Andere Bezeichnung: Alpha-1 Microglobulin (AMBP (AMBP Produkte)

Synonyme: A1M ELISA Kit, EDC1 ELISA Kit, HI30 ELISA Kit, IATIL ELISA Kit, ITI ELISA Kit, ITIL ELISA Kit, ITILC ELISA Kit, UTI ELISA Kit, ambp ELISA Kit, MGC64242 ELISA Kit, AMBP ELISA Kit, DKFZp470D2211 ELISA Kit, AI194774 ELISA Kit, ASPI ELISA Kit, HI-30 ELISA Kit, Intin4 ELISA Kit, Itil ELISA Kit, ambpl ELISA Kit, zgc:66321 ELISA Kit, alpha-1-microglobulin/bikunin precursor ELISA Kit, alpha-1-microglobulin/bikunin precursor L homeolog ELISA Kit, alpha 1 microglobulin/bikunin ELISA Kit, AMBP ELISA Kit, Ambp ELISA Kit, ambp.L ELISA Kit, ambp ELISA Kit

UniProt: P02760