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LMAN2 ELISA Kit

LMAN2 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730685
  • Target Alle LMAN2 ELISA Kits anzeigen
    LMAN2 (Lectin, Mannose-Binding 2 (LMAN2))
    Reaktivität
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human LMAN2 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human LMAN2.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product LMAN2 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle LMAN2 ELISA Kits anzeigen
    LMAN2 (Lectin, Mannose-Binding 2 (LMAN2))
    Andere Bezeichnung
    LMAN2 (LMAN2 Produkte)
    Synonyme
    Lman2 ELISA Kit, wu:fb16f04 ELISA Kit, zgc:158761 ELISA Kit, C5orf8 ELISA Kit, GP36B ELISA Kit, VIP36 ELISA Kit, 1110003H06Rik ELISA Kit, 1300009F09Rik ELISA Kit, AA408240 ELISA Kit, AL023023 ELISA Kit, AU040819 ELISA Kit, Vip36 ELISA Kit, lectin, mannose binding 2 ELISA Kit, lectin, mannose-binding 2 ELISA Kit, LMAN2 ELISA Kit, lman2 ELISA Kit, Lman2 ELISA Kit
    UniProt
    Q12907
    Pathways
    SARS-CoV-2 Protein Interaktom
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