FABP4 ELISA Kit

Produktnummer ABIN6730639

Produktdetails FABP4 ELISA Kit

Target: FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse FABP4/A-FABP ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse FABP4.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu FABP4

Target: FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))

Andere Bezeichnung: FABP4 (A-FABP (FABP4 Produkte)

Synonyme: A-FABP ELISA Kit, AFABP ELISA Kit, ALBP ELISA Kit, aP2 ELISA Kit, LOC100223994 ELISA Kit, FABP ELISA Kit, AP2 ELISA Kit, FABP3 ELISA Kit, FABP4 ELISA Kit, MGC84940 ELISA Kit, fabp4 ELISA Kit, 422/aP2 ELISA Kit, ALBP/Ap2 ELISA Kit, Ap2 ELISA Kit, Lbpl ELISA Kit, Albp ELISA Kit, fatty acid binding protein 4 ELISA Kit, fatty acid-binding protein, adipocyte ELISA Kit, fatty acid binding protein 4, adipocyte ELISA Kit, fatty acid binding protein 4, adipocyte L homeolog ELISA Kit, Fatty acid-binding protein, adipocyte ELISA Kit, adipocyte fatty acid-binding protein ELISA Kit, adipocyte fatty acid binding protein ELISA Kit, FABP4 ELISA Kit, LOC100223994 ELISA Kit, fabp4.L ELISA Kit, fabp4 ELISA Kit, Fabp4 ELISA Kit, LOC100732353 ELISA Kit, LOC100861279 ELISA Kit, LOC100057425 ELISA Kit, LOC395224 ELISA Kit

UniProt: P04117

Pathways: Brown Fat Cell Differentiation