CD22 ELISA Kit (CD22 Molecule)

Details for Product CD22 ELISA Kit No. ABIN6730583
Antigen
  • A530093D23
  • Lyb-8
  • Lyb8
  • SIGLEC-2
  • SIGLEC2
  • siglec-2
  • CD22 antigen
  • CD22 molecule
  • Cd22
  • CD22
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
6
4
1
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Optionen
Verwendungszweck Mouse Siglec-2 ELISA Kit.
Proben Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität This ELISA antibody pair recognizes Mouse Siglec-2.
Produktmerkmale
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmide, Primers & weitere CD22 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Siglec-2 (CD22 ELISA Kit Abstract)
UniProt P35329
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Protokoll
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Beschränkungen Nur für Forschungszwecke einsetzbar
Informationen zur Lagerung The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit 6 months