CTSC ELISA Kit

Produktnummer ABIN6730547

Produktdetails CTSC ELISA Kit

Target: CTSC (Cathepsin C (CTSC))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse Cathepsin C/DPPI ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse Cathepsin C.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CTSC

Target: CTSC (Cathepsin C (CTSC))

Andere Bezeichnung: Cathepsin C (DPPI (CTSC Produkte)

Synonyme: CPPI ELISA Kit, DPP-I ELISA Kit, DPP1 ELISA Kit, DPPI ELISA Kit, HMS ELISA Kit, JP ELISA Kit, JPD ELISA Kit, PALS ELISA Kit, PDON1 ELISA Kit, PLS ELISA Kit, MGC69126 ELISA Kit, MGC107782 ELISA Kit, AI047818 ELISA Kit, CATC ELISA Kit, cb912 ELISA Kit, ik:tdsubc_1h2 ELISA Kit, sb:cb146 ELISA Kit, wu:fb34g12 ELISA Kit, wu:fj58d01 ELISA Kit, cathepsin C ELISA Kit, cathepsin C L homeolog ELISA Kit, cathepsin CPC1 ELISA Kit, CTSC ELISA Kit, ctsc.L ELISA Kit, ctsc ELISA Kit, TP03_0357 ELISA Kit, CMU_003360 ELISA Kit, CPC1 ELISA Kit, NAEGRDRAFT_80477 ELISA Kit, NAEGRDRAFT_37577 ELISA Kit, Ctsc ELISA Kit, Smp_183330 ELISA Kit

UniProt: P97821