Follistatin ELISA Kit
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- Target Alle Follistatin (FST) ELISA Kits anzeigen
- Follistatin (FST)
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Mouse Follistatin ELISA Kit.
- Proben
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair recognizes Mouse Follistatin.
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product FST ELISA Kit
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Follistatin (FST) ELISA Kits anzeigen
- Follistatin (FST)
- Andere Bezeichnung
- Follistatin (FST Produkte)
- Synonyme
- CG12955 ELISA Kit, CG12956 ELISA Kit, CG33466 ELISA Kit, Dmel\\CG33466 ELISA Kit, Fol1 ELISA Kit, dFS ELISA Kit, dFol1 ELISA Kit, dfs ELISA Kit, fs ELISA Kit, FS ELISA Kit, foll ELISA Kit, xfs ELISA Kit, FST ELISA Kit, fst ELISA Kit, AL033346 ELISA Kit, FOL1 ELISA Kit, Fst-288 ELISA Kit, RATFOL1 ELISA Kit, cb446 ELISA Kit, fd07h10 ELISA Kit, fst1 ELISA Kit, wu:fd07h10 ELISA Kit, Follistatin ELISA Kit, follistatin ELISA Kit, follistatin L homeolog ELISA Kit, follistatin a ELISA Kit, Fs ELISA Kit, fst ELISA Kit, FST ELISA Kit, LOC100141662 ELISA Kit, fst.L ELISA Kit, Fst ELISA Kit, fsta ELISA Kit
- UniProt
- P47931
- Pathways
- Negative Regulation of Hormone Secretion
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