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GALP ELISA Kit (Galanin-Like Peptide) ELISA Kit

GALP Reaktivität: Human, Maus, Ratte Colorimetric Competition ELISA 0.1 ng/mL - 1000 ng/mL Cell Culture Supernatant, Serum
Produktnummer ABIN6730484
$501.40
Zzgl. Versandkosten $45.00
96 tests
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 2 bis 3 Werktagen
  • Target
    GALP
    Reaktivität
    Human, Maus, Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    0.1 ng/mL - 1000 ng/mL
    Untere Nachweisgrenze
    0.1 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human/Mouse/Rat Galanin-like peptide EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.
    Proben
    Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This EIA kit is designed to detect human, mouse, and rat Galanin-like peptide
    Sensitivität
    2.4 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Aufbereitung der Reagenzien

    Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Haltbarkeit
    6 months
  • Target
    GALP
    Andere Bezeichnung
    Galanin-like peptide (GALP ELISA Kit Abstract)
    Synonyme
    GALP, galanin like peptide, galanin-like peptide, GALP, ci-galp, Galp
    Gen-ID
    85569
    UniProt
    Q9UBC7
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