DDC ELISA Kit

Produktnummer ABIN6730403

Produktdetails DDC ELISA Kit

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse DDC ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse DDC.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu DDC

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Andere Bezeichnung: DDC (DDC Produkte)

Synonyme: AADC ELISA Kit, wu:fa56d05 ELISA Kit, wu:fd59h03 ELISA Kit, wu:fk20h01 ELISA Kit, zgc:65801 ELISA Kit, zgc:76929 ELISA Kit, Ddc ELISA Kit, DdcDv ELISA Kit, Dvir\\GJ17995 ELISA Kit, GJ17995 ELISA Kit, dvir_GLEANR_2561 ELISA Kit, Aadc ELISA Kit, 5-HT ELISA Kit, CG10697 ELISA Kit, DDC ELISA Kit, DdcDm ELISA Kit, Dmel\\CG10697 ELISA Kit, ddc ELISA Kit, fDDC ELISA Kit, l(2)37Bl ELISA Kit, l(2)37Ch ELISA Kit, l(2)k02104 ELISA Kit, dopa decarboxylase ELISA Kit, Dopa-decarboxylase ELISA Kit, dopa decarboxylase (aromatic L-amino acid decarboxylase) ELISA Kit, Dopa decarboxylase ELISA Kit, dopa decarboxylase L homeolog ELISA Kit, DDC ELISA Kit, ddc ELISA Kit, Ddc ELISA Kit, Dvir\Ddc ELISA Kit, ddc.L ELISA Kit

UniProt: O88533

Pathways: Dopaminergic Neurogenesis