CD73 ELISA Kit

Produktnummer ABIN6730402

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CD73.

Kurzübersicht für CD73 ELISA Kit (ABIN6730402)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7 pg/mL - 1500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails CD73 ELISA Kit

Untere Nachweisgrenze: 7 pg/mL

Verwendungszweck: Mouse 5'-Nucleotidase/CD73 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse 5'-Nucleotidase.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Reagenzien:

Recommended Dilution for serum and plasma samples100 fold

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung: 5'-Nucleotidase (CD73

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process