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UCHL1 ELISA Kit
UCHL1
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730322
Produktdetails UCHL1 ELISA Kit
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Target
Alle UCHL1 ELISA Kits anzeigen
UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
Reaktivität
Alle Reaktivitäten für UCHL1 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse UCHL1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse UCHL1.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu UCHL1
(ausblenden)
Target
Alle UCHL1 ELISA Kits anzeigen
UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
Andere Bezeichnung
UCHL1 (UCHL1 Produkte )
Synonyme
PARK5 ELISA Kit, PGP 9.5 ELISA Kit, PGP9.5 ELISA Kit, PGP95 ELISA Kit, Uch-L1 ELISA Kit, UCH-L1 ELISA Kit, UCHL1 ELISA Kit, cb358 ELISA Kit, wu:fc55h08 ELISA Kit, park5 ELISA Kit, pgp9.5 ELISA Kit, uch-l1 ELISA Kit, MGC132191 ELISA Kit, uchl1 ELISA Kit, AW822034 ELISA Kit, C88048 ELISA Kit, R75593 ELISA Kit, UCHL-1 ELISA Kit, gad ELISA Kit, ubiquitin C-terminal hydrolase L1 ELISA Kit, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) ELISA Kit, ubiquitin C-terminal hydrolase L1 L homeolog ELISA Kit, ubiquitin carboxyl-terminal esterase L1 ELISA Kit, ubiquitin carboxy-terminal hydrolase L1 ELISA Kit, UCHL1 ELISA Kit, uchl1 ELISA Kit, uchl1.L ELISA Kit, LOC100304750 ELISA Kit, Uchl1 ELISA Kit
UniProt
Q9R0P9
Pathways
Feeding Behaviour
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